Identification of New Amylolytic Enzymes from Marine Symbiotic Bacteria of Bacillus Species

نویسندگان

چکیده

α-amylases are one of the most common and important industrial enzymes widely used in various industries. The present study was conducted with aim isolating identifying symbiotic α-amylase enzyme-producing bacteria intestine Silago Sihama Rasterliger Canagorta fish living Qeshm Island, Hormozgan. intestinal these species were isolated using nutrient agar culture medium; then, producing screened a special medium containing starch Lugol’s solution test. enzyme activity measured substrate. Finally, highest selected identified by 16S rRNA gene sequence analysis. results showed that out 22 bacteria, 10 able to grow medium, 5 strains had ability produce relatively stronger halos. four bacterial belonging genus Bacillus registered NCBI database as B. subtilis HR13, HR14, HR15, HR16. Among strains, two subtilis, HR13 HR16, displayed high maximum at 60 °C pH values 7, respectively. α-Amylase from marine can be considered potential candidates for application

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ژورنال

عنوان ژورنال: Catalysts

سال: 2023

ISSN: ['2073-4344']

DOI: https://doi.org/10.3390/catal13010183